Gvulot (Template:Lang-he-n, lit. Borders) is a kibbutz in southern Israel. Located in the north-western Negev desert, it falls under the jurisdiction of Eshkol Regional Council. In 2006 it had a population of 239.
The settlement was established on land owned by the Jewish National Fund 12 May 1943 by immigrants from Romania and Turkey who were members of the "Kibbutz Eretz Israel Gimel" group of Hashomer Hatzair. Initially named Mitzpe Gvulot (Template:Lang-he, lit. Borders lookout), it was the first of the three lookouts, the others being Beit Eshel and Revivim, and was the first modern Jewish settlement in the Negev. Its purpose was to guard JNF land, as well as to research the soil and climate of the region and assess their suitability for agriculture.
It was recognised as a kibbutz in 1946, and moved around 1.5 kilometres south to its present location in 1949.